Journal: Viruses

Article Title: An Improved αvβ6-Receptor-Expressing Suspension Cell Line for Foot-and-Mouth Disease Vaccine Production

doi: 10.3390/v14030621

Figure Lengend Snippet: Flow cytometry analyses of integrin and heparan sulphate expression on the cell surface of BHK-21 cell lines. Four adherent cell lines (BHK-21 Strain 31, 35 and 38, and BHK 21 Clone 13), and the respective suspension cell lines generated from them (SP Strain 31, 35, and 38, and SP Clone 13) were analyzed by flow cytometry using a panel of antibodies recognizing α5β1, β1, αvβ3, αvβ5, αvβ6, αvβ8 and heparan sulphate. BHK21C13-2P was analyzed alongside and served as a comparative suspension cell line. Matched isotype antibodies served as negative controls and the expression of each receptor is presented as the percentage of positive cells.

Article Snippet: To develop an improved suspension BHK-21 cell line permissive to infection by both cell culture adapted as well as non-cell culture-adapted FMD vaccine strains, four BHK-21 adherent cell lines were obtained from the European Collection of Authenticated Cell Cultures (ECACC) and propagated with agitation until they were adapted to suspension (SP) culture; the four cell lines were BHK 21 Strain 31 (ECACC 93120840), BHK 21 Strain 35 (ECACC 93120841), BHK 21 Strain 38 (ECACC 93120842) and BHK 21 Clone 13 (ECACC 85011433).

Techniques: Flow Cytometry, Expressing, Suspension, Generated, Comparison, Control